Tares the current absorbance output to 0.0000 AU. Use this after the baseline has stabilized with the mobile phase flowing. [LAMP]: Turns the Deuterium ( D2cap D sub 2 ) or Tungsten ( ) lamps ON or OFF manually. [ENTER]: Confirms value entries and menu selections. 4. Standard Operating Procedure (SOP) Startup Sequence Turn on the main power switch of the SPD-20A. Press the [LAMP] key to ignite the D2cap D sub 2
Utilize the built-in mercury lamp calibration feature to ensure the 190-700 nm range is accurate. B. Dual-Wavelength Measurement
Before operating the SPD-20A, it is critical to adhere to safety guidelines:
provides a practical guide on how to integrate the SPD-20A into a full HPLC workflow. Community Troubleshooting : Platforms like Chromatography Forum shimadzu spd20a user manual
The appropriate lamp must be selected for the measurement wavelength:
If baseline noise increases or phantom peaks appear, the flow cell may need flushing with an appropriate solvent (e.g., methanol, water, or 1M nitric acid for specific contaminants). Lamp Replacement: The D2cap D sub 2
The SPD-20A balances high sensitivity with long-term stability. Understanding its core specifications helps optimize performance. Deuterium ( D2cap D sub 2 Wavelength Range: 190 nm to 700 nm. Spectral Bandwidth: 8 nm. Wavelength Accuracy: ±1 nm. Wavelength Reproducibility: ±0.1 nm. Noise Level: AU (under specified conditions). Drift: AU/hour (under specified conditions). 2. Installation and Initial Setup Tares the current absorbance output to 0
Allow the lamp to warm up for to stabilize the baseline. Step 2: Setting Parameters
If you need help resolving a specific error code on your , or if you want to walk through step-by-step diagnostic tests , please let me know. Share public link
190–700 nm (SPD-20A), 190–900 nm (SPD-20AV). [ENTER]: Confirms value entries and menu selections
This informative paper is intended for educational and quick-reference purposes only. Always consult the original manufacturer manual before performing maintenance or repairs.
One of the key advantages of the SPD-20A is its ability to monitor two wavelengths simultaneously.
If managing via the keypad, press FUNC until LAMP appears, select ON , and press ENTER . Allow the D2cap D sub 2
Connect the tubing coming from the column outlet directly to the bottom port of the flow cell. Use standard 1/16-inch PEEK or stainless steel fittings.